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Use of molecular typing methods in characterizing MRSA infections in Ohio- [e-book]
Use of molecular typing methods in characterizing MRSA infections in Ohio- [e-book]
- 자료유형
- 학위논문
- ISBN
- 9781339523439
- 서명/저자
- Use of molecular typing methods in characterizing MRSA infections in Ohio - [e-book]
- 발행사항
- Ann Arbor, MI : UMI Dissertations Publishing, 2015
- 형태사항
- 244 p
- 주기사항
- Adviser: Kurt B. Stevenson.
- 학위논문주기
- Thesis (Ph.D.) - The Ohio State University, 2015.
- 초록/해제
- 요약 : Methicillin-resistant Staphylococcus aureus (MRSA) is a genetically heterogeneous bacterial organism responsible for causing significant human disease. Molecular strain typing of MRSA is regularly implemented to enhance surveillance procedures, supplement outbreak control programs, and to characterize evolutionary patterns of MRSA. Numerous molecular strain typing methods have been developed to characterize MRSA, but all have significant advantages and disadvantages. Four of the more commonly used typing methods include pulsed field gel electrophoresis (PFGE), Staphylococcal protein A (spa) typing, repetitive element PCR (rep-PCR) typing, and Staphylococcal chromosome cassette mec (SCC mec) typing.
- 초록/해제
- 요약 : The objective of this dissertation was to investigate the use of molecular typing methods in characterizing MRSA using a set of isolates collected from eight hospitals in Ohio from 2007 through 2010. Our three specific aims were: 1.) To evaluate the discriminatory power and concordance of rep-PCR, spa, SCCmec and PFGE typing in the molecular characterization of MRSA 2.) To characterize the association between spa type assignment and MRSA infection presentation and 3.) To describe the distribution and diversity of MRSA isolate strain types across seven community hospitals in Ohio collected over a one year period. In our study we found that rep-PCR typing had the highest discriminatory power of the four typing methods, and that there was high directional concordance between rep-PCR typing and spa typing, PFGE typing, and SCCmec typing. spa typing also exhibited high directional concordance with PFGE and SCCmec typing. We also found a lack of independence between spa type assignment and MRSA specimen type. Specifically, isolates assigned to spa type 2 were more likely to come from a blood specimen than a skin/soft tissue specimen, and isolates assigned to spa type 1 were less likely to come from either a blood specimen or a sputum specimen than from a skin/soft tissue specimen. Finally, we found that two rep-PCR types persisted at different time intervals across all seven community hospitals, and that there was high rep-PCR type diversity among our isolates at each hospital. We also found evidence that rep-PCR typing might be useful in detecting outbreak strains. There were two spa types that persisted across also seven hospitals across the entire study period, and there was moderate diversity in spa type assignment among the isolates at each hospital. These differences give some support to using rep-PCR typing over spa typing for short term MRSA surveillance.
- 초록/해제
- 요약 : Molecular strain typing is a ubiquitous tool for characterizing MRSA, but there are a number of different typing methods available to use. Our results suggest that rep-PCR typing is highly concordant with other popular typing methods and is a useful tool for short term surveillance. We also found that spa typing is associated with the manifestation of an MRSA infection. Future studies should focus on correlating these strain type patterns to virulence factors associated with persistence and pathogenesis.
- 기타 저자
- The Ohio State University Public Health
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- Control Number
- chimsin:470110
소장정보
- 예약
- 서가에 없는 책 신고
- 보존서고 도서대출신청
- 나의폴더
- 우선정리요청
등록번호 | 청구기호 | 소장처 | 대출가능여부 | 대출정보 |
---|---|---|---|---|
T0013033 | DT Ph.D. | 전자화일 | 열람만 가능 |
열람만 가능 마이폴더 |
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